JCSG Crystallisation Screen Series
- Available 1.7 ml 96 deep-well block formats
- Manufactured by Rigaku Reagents
- Includes the JCSG Core Series, JCSG + and JCSG Top 96
- Resulting from the analysis of 500,000 crystallisation experiments
- Sparse Matrix screening
The Joint Centre for Structural Genomics formatted the JCSG Core Suite screens as a result of analysing over 500,000 high-throughput crystallization experiments, performed at the JCSG. The 384 crystallization formulations were selected based on the highest hit rates in initial screening.
JCSG Top96 includes the 96 conditions with the highest number of hits.
The Wizard JCSG+™ 96 well block plate sparse matrix protein crystallization screen is designed for coarse screening of initial crystallization and solubility conditions for biological macromolecules. Researchers from the Joint Centre for Structural Genomics (JCSG) formulated this screen with the goal of maximizing the coverage of the crystallization parameter space (buffers, salts, precipitants, and pH) and eliminating redundancy. The buffers give final solution pH between 4.2 and 10.5. Precipitants include volatile reagents such as ethanol, high molecular weight polymers such as PEG-8000, and salts such as ammonium sulphate.
96-Well Block Plate formulations are 1.7 ml each in a 96 deep-well matrix block plate. All formulations are prepared with ASTM type-1 water and high-purity chemicals, 0.2 µm filtered in sterile packaging.
Links to Instruction, Formulation and MSDS Sheets
Item: JCSG Core Suite 1, 96 formulations, 1.7 ml per well.
Item: JCSG Core Suite 2, 96 formulations, 1.7 ml per well.
Item: JCSG Core Suite 3, 96 formulations, 1.7 ml per well.
Item: JCSG Core Suite 4, 96 formulations, 1.7 ml per well.
Item: Wizard: JCSG+ Screen, 96 conditions, 1.7 ml per well.
Item: JCSG Top96, 96 formulations; 1.7 ml per well.
Item: JCSG Top96 Cryo, 96 formulations; 1.7 ml per well.