An innovative support for serial data collection from micro-crystals
The SWISSMX CM supports are designed for high throughput serial data collection from micro-crystals at synchrotrons and XFELS. SWISSMX CMs are designed and engineered by scientists of the Macromolecular Crystallography group and the Laboratory of Micro and Nanotechnology at the Paul Scherrer Institute in collaboration with the Institute of Polymer Nanotechnology at Fachhochschule Nordwestschweiz. The polymer-based ultra-thin porous membranes present users with an effective and accessible product for use at MX beamlines.
Low Background Ultra Thin Porous Membranes
Amorphous COC (Cyclic Olefin Copolymer) for low X-ray scattering
Microfabricated film with imprinted perforations
Easy deposition of drop-scale samples
Efficient blotting to minimize background
Hydrophilic surface properties help to retain a thin hydration film around the crystals
Designed to be used at cryogenic temperatures
Dimensions designed for fitting in standard (cryo)streams
Flash-cooling possible in cryojet or liquid nitrogen
Room temperature use possible: compatible with humidifying jets, enclosure possible
SPINE base or ALS style base
Compatible with Uni-puck and with SPINE pucks (cryovial)
Compatible with a number of sample changers including the new TELL at Swiss Light Source and SwissFEL SwissMX
High transparency for excellent visibility and imaging
SWISSCI crystallisation plates are available in 3 polymers. Polystyrene (PS) is a cost-effective polymer that Is optically clear when using standard bright field or visible light sources. However, when imaging drops using both cross polarised (XP) and UV light, the quality of the image is impaired by the interaction of the polymer with the light source.
SWISSCI’s UVP polymer has been designed to reduce the auto-fluorescence coming from the plate, improving the UV image quality.
SWISSCI’s UVXPO polymer also has the same low auto-fluorescence while also showing uniform zero background when imaging using cross-polarised light. This makes it our most advanced polymer to date.
SWISSCI’s UVXPO Sealing sheets are a 50 µm film with 50 µm inert encapsulated silicone adhesive. They are optically clear, with minimal to no auto-fluorescence (UV compatible), and are especially suited for protein crystallization and qPCR applications.
The encapsulated silicone adhesive is non-tacky until pressed against the sealing surface, at which time adhesive is released only in sealing areas to form the strongest available heat-resistant seal around each well on the plate. The adhesive on non-sealing areas of the film, such as directly over sample wells, remains encapsulated and inert.
UVXPO Sealing sheets are sized to fit within the edges of SWISSCI plates. Two end tabs assist in positioning the film on the plate, and the non-tacky adhesive surface simplifies handling. Easy removal of the end tabs at perforated boundaries prevents lifting and higher evaporation rates that can occur with films that overlap the plate rim.
· High optical clarity · Minimal to no auto-fluorescence (UV compatible) · Chemically inert; no extractables except at extreme pH · DMSO resistant for HTS · Heat resistant recommended for temperatures from -70 °C to +105 °C · Certified DNase-, RNase-, and nucleic-acid-free · Silicone adhesive forms the strongest available seal for evaporation prevention · Non-tacky adhesive layer simplifies handling of the film prior to sealing
Order Details: UVXPO Sealing sheets – Box of 100 – Code: UVXPO-SEAL
The new SWISSCI XPol LCP allows excellent crystal viewing for even the smallest of crystals, with uniform zero background when using cross polarised light.
The SWISSCI XPol LCP was developed closely with the Protein Crystallisation Center of the University of Zurich. The center has many years of expertise in crystallizing membrane proteins. The XPol LCP kit has been specially formulated to improve performance versus standard LCP systems.
The Hydrophobic (silanized) Glass Base comes in ANSI/SLAS 1-2004-Standard (SBS) format allowing for easy automation and high throughput screening. The Double-Sided Pressure Adhesive, available in 60μm and 120μm, comes readily stuck to the Glass Base with space for barcode application. The Double-Sided Pressure Adhesive has 96 holes which are 7mm in diameter and have a 2.3μ volume in the 60μm version and 4.6μl volume for 120μm. The large diameter almost eliminates the occurrence of precipitation solution being pulled to the side, which can often happen with smaller diameter holes.
The SWISSCI XPol LCP comes as standard with 175μm Cover Glass. For the addition of UV/Flu, UV Cover Films are also available upon request at 40μm, 100μm or 200μm thickness.
After great success with the launch of the 2-Lens UVXPO Plate, SWISSCI are pleased to announce our 3-Lens Midi Plates are now also available in UVXPO, Product
UVXPO is a new polymer mixture specially formulated so that crystals can be viewed in UV, Polar, and Visible light. UVXPO is short for UV compatible as well as excelling uniform (zero) background when using cross polarised light.
The SWISSCI LCP Modular range aims to offer more flexibility to the membrane protein crystallographers LCP experiment. There are three choices of LCP Bases which are combined with one of five pre-affixed LCP Double Sided Pressure Adhesives*. The Pressure Adhesives are of varying thickness and well dimensions. To complete the LCP sandwich plate there is a choice between two sizes of Cover Glass or three UVP Cover Films that are of varying thickness.